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celltechnology NADPH 100 說明書

更新時間：2017-08-02 點(diǎn)擊次數(shù)：2444

世界*實(shí)驗(yàn)材料供應(yīng)商 Cell Technology正式授權(quán)上海起發(fā)為其中國代理， Cell Technology在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來的產(chǎn)品，的服務(wù)，簽約 Cell Technology就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Cell Technology成立于1998年，致力于為研究院、生物技術(shù)公司及制藥企業(yè)的科研人員研發(fā)生產(chǎn)*的細(xì)胞功能研究用試劑與試劑盒。這些檢測試劑與試劑盒可以實(shí)現(xiàn)不破壞細(xì)胞形態(tài)和結(jié)構(gòu)的情況下原位檢測細(xì)胞成分，研究細(xì)胞內(nèi)發(fā)生的特定事件.Cell Technology發(fā)布了通過測定胞內(nèi)活性caspase及線粒體膜電位研究細(xì)胞凋亡的一系列試劑。隨著公司研發(fā)團(tuán)隊(duì)的持續(xù)努力，我們又推出了一系列高特異性的caspase檢測試劑，以及cathepsin檢測的試劑和ELISA試劑盒。目前Cell Technology提供的檢測試劑和ELISA試劑盒涵蓋Caspase、NOS、ROS、線粒體膜電位、信號通路和蛋白磷酸化等多個研究領(lǐng)域，產(chǎn)品倍各大高校、研究機(jī)構(gòu)、生物技術(shù)企業(yè)和制藥企業(yè)采用。

Cell Technology主要產(chǎn)品簡介

1、Fluoro Cholesterol：高靈敏、一步檢測生物樣本中總膽固醇含量，可用于細(xì)胞代謝與藥物作用的研究。

2、Fluoro NADP：檢測組織或細(xì)胞提取液內(nèi)NADP/NADPH含量，與NAD/NADH檢測無交叉，可用于細(xì)胞死亡、抗氧化/氧化應(yīng)激、能量代謝及線粒體功能研究。

3、Fluoro Sarcosine：定量檢測細(xì)胞、血清及組織提取物中的肌氨酸含量。

4、Fluoro IndoBlu：定量檢測細(xì)胞中IndoBlu的含量，適用于細(xì)胞增殖研究。

5、Fluoro Lactate：定量檢測生物樣本中 L-lactate的含量。

6、Fluoro Phosphate：定量檢測生物樣本中的磷含量，靈敏度可達(dá)200nM。

Fluoro NADP

Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples

產(chǎn)品編號:NADPH 100-2
產(chǎn)品名稱:Fluoro NADP™ Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples/熒光NADP/NADPH檢測試劑盒
產(chǎn)品規(guī)格:100Test

Fluoro NADP™ NEW!
Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples

Key Benefits:

Detection of NADP/NADPH activity in cells or tissue extracts
Detection of NADP/NADPH levels in antioxidation and oxidative stress
Detection of NADP/NADPH levels in cell death, energy metabolism, and mitochondria function
Species Independent - NADP/NADPH detection in Bacterial, fungal and plant cells
Highly Sensitive – Detects up to 4nM NADP and NADPH
Highly Specific - No Cross reactivity with NAD/NADH
Easy to Use – 96 well Fluorescent Plate reader readout

Introduction

Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells.

NADPH is produced in the oxidative phase of the pentose phosphate pathway in cells, a multifunctional pathway whose primary purpose is to generate reducing power in the form of NADPH. NADPH is a cofactor for enzymes that synthesize energy-rich molecules and provide the reducing equivalents for the oxidation-reduction involved in protecting the cell from the toxicity of reactive oxygen species (ROS) and NADPH oxidase-dependent ROS generation. Both NAD and NADP have been shown to influence hemoglobin affinity for oxygen in erythrocytes. In plant cells, NADPH is used as the reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis (1-2).

Cell Technology’s Fluoro NADP/NADPH provides a highly reliable, sensitive fluorometric assay for the quantification of NADP, NADPH and their ratio in biological samples.

Assay Principle

The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is oxidized in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH.

Reaction:

1. NADPH + non-fluorescent detection reagent + electron coupler → fluorescent analog + NADP

2. NADP + enzyme coupled reaction → NADPH (then proceeds to reaction 1).

Excitation: 530-570nm and Emission at 590-600nm

References

1. Comprehensive Invited Review WEIHAI YING. NAD /NADH and NADP /NADPH in Cellular Functions and Cell Death: Regulation and Biological Consequences. ANTIOXIDANTS &REDOX SIGNALING Volume 10,Number 2,2008.

2. Bedard K and Krause KH. The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.Physiol Rev 87:245 –313,2007.

3. Lowry, Oliver H., Passonneau, Janet V. and Rock, Martha K. The Stability of Pyridine Nucleotides. The Journal of Biological Chemistry, 236, #10, 1961.

Kit contents (for 100 assays)

1. Enzyme Mix 170μL    Part # 6022 .......................... 4-8C

2. NADP/NADPH Lysis Solution 12.5mL Part# 3050 ............................4-8C

3. NADP Extraction Buffer 22 mL Part# 3046 (Green label) ..........4-8C

4. NADPH Extraction Buffer 22 mL Part# 3047 (Magenta label)........4-8C

5. Reaction Buffer 11 mL. Part# 3049 ............................4-8C

6. Standard curve diluent 15 ml. Part # 3052............................4-8C

7. NADPH Detection reagent 110 μL    Part # 4019...........................-20C

8. 3X Substrate Mix 6mL Part # 3048........................... -20C

9. NADPH Standard- 3 vials dried   Part# 7014.............................-20C

The following kits are available:
Catalog #	Size	Price (US$)
NADPH 100-2	100 Tests	6930.00

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5：良好的信譽(yù)，大部分客戶我們提供貨到付款服務(wù)，客戶包括清華，北大交大復(fù)旦，中山等100多所大學(xué)，ROCHE，阿斯利康，國藥，fisher等500多家公司

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